Reproduction Abstracts

SCNT technology provides potential applications for biomedical and agriculture. However, the efficiency of SCNT is still low. Incomplete epigenetic reprogramming of transferred somatic cell is believed to be one of main causes of developmental problems. Hanwoo is considered as only bovine species with mainly reddish color but quite a few of mini-, white-colored, black-colored, striped cows. The objective of this study was to produce cloned offspring from endangered mini-Hanwoo...

Stem cells can provide a useful tool for studying on mechanisms of embryonic development and production of transgenic animal. Although somatic cells are able to convert into reprogrammed cells by introduction of exogenous reprogramming factors, however, the efficiency of iPSC is still low. Murine somatic cells without transfection of exogenous transcription factors were easily able to be reprogrammed by stimulus of low pH. Here we attempted whether to convert bFFs into pluripo...

piPS cells are divided into naïve and primed states. These states may depend on culture conditions with/without cytokines. Only piPS cells of naïve state have the capacity for producing chimeric offspring and long term maintenance of pluripotency. The objective of this study was to generate piPS cells of naïve state under culture conditions with 2i of the Erk/2 and of GSK3. PFFs were transduced with SIX reprogramming factors. After the transduction piPS cells we...